In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. The EVOM3 uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluence in cell cultures. The EVOM3 produces a low AC current that avoids electrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. The EVOM3 qualitatively measures cell monolayer health and quantitatively measures cell confluence. Keyed electrode base for repeatable placement gives more consistent results, eliminating the need for multiple readings.Ĭonfluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using the unique electronic circuit of the EVOM3 and the new STX2-PLUS electrode.The STX2, STX3 and all STX100s require the use of this adapter with the EVOM3. NOTE: A 99672 EVOM2 to EVOM3 Electrode Adapter is sold separately. System Components What is included with the EVOM3ģ00749 USB drive 32 GB (Used for storage. Also contains a Python 3.8 program for continuous digital monitoring of a target insert).ĩ9673 Calibration kit, 1000Ω Test Resistor Shielded cable to minimize electrical and cell phone interference.Weighted self-standing electrode for hands-free stable measurements.STX2-PLUS new electrode designed for 12 and 24-well plates.The new shielded electrodes are now designed to minimize electrical interference and to be more easily maintained. It is location re-placeable in the insert for repeatable and consistent measurements. The STX2-PLUS electrode was designed for easy insertion into many 24-well plates. The EVOM3 has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistance ranges up to 100 KΩ. The auto ranging resistance feature allows for fast resistance measurements, and an over-range display feature eliminates false readings. When used with the footswitch it enables handsfree recording of measurements.Īt the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readings due to its fast stabilization, automatic twenty times sampling average and low noise design. The touch screen interface provides users an intuitive, easy-to-use menu for configuration.Įliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSV format for easy transfer to spreadsheets and data analysis programs. The new graphical displays for trend analysis and measurement values helps scientists deliver simple, stepwise methodology during experimental measurements. The EVOM3 delivers improved workflow efficiency, more stable and repeatable measurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters. Providing users with vital feedback during experiment measurements, the EVOM3’s large screen offers a range of informational views.
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